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Research Methodology & Design Research

Browse the latest research summaries in the field of research methodology & design for spinal cord injury patients and caregivers.

Showing 611-620 of 702 results

Medical ImagingBioinformaticsResearch Methodology & Design

On the acceptance of “fake” histopathology: A study on frozen sections optimized with deep learning

Journal of Pathology Informatics, 2022 • December 20, 2022

This study investigates the use of generative adversarial networks (GANs) to enhance the quality of frozen section images by translating them into virtual paraffin sections, aiming to improve diagnost...

KEY FINDING: Pathologists' detection accuracy of virtual paraffin sections was between 0.62 and 0.97, indicating a challenge in distinguishing real from AI-generated images.

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Regenerative MedicineImmunologyResearch Methodology & Design

Decoding the proregenerative competence of regulatory T cells through complex tissue regeneration in zebrafish

Clin Exp Immunol, 2021 • September 7, 2021

This review explores the proregenerative roles of Tregs in zebrafish and mammals, highlighting the FoxP3-dependent immunosuppressive function of Tregs in zebrafish as a useful model for future researc...

KEY FINDING: Zebrafish possess two FOXP3 orthologs, foxp3a and foxp3b, potentially serving a redundant role in Treg formation and activity.

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Regenerative MedicineGeneticsResearch Methodology & Design

Induced Pluripotent Stem Cells (iPSCs)—Roles in Regenerative Therapies, Disease Modelling and Drug Screening

Cells, 2021 • September 5, 2021

Human iPSCs have become a powerful tool in basic as well as translational and clinical research because of their ability to be maintained indefinitely whilst preserving the genetic makeup of the host. ...

KEY FINDING: iPSC-based disease models have been studied for cardiac channelopathies including hereditary long QT syndrome (LQTS), dilated cardiomyopathy (DCM), hypertrophic cardiomyopathy (HCM), and arrhythmogenic right ventricular cardiomyopathy (ARVC).

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OncologyResearch Methodology & Design

Construction and validation of nomograms for non‑metastatic Ewing sarcoma: A prognostic factor analysis based on the SEER database

ONCOLOGY LETTERS, 2021 • January 1, 2021

The present study aimed to describe the clinical characteris­tics, and to construct and validate nomograms for patients with non‑metastatic Ewing sarcoma. Age, sex, tumor extension and surgery were in...

KEY FINDING: Age, tumor extension, sex, International Classification of Diseases for Oncology, 3rd Edition histology, surgery and chemotherapy were identified as independent risk factors for OS and CSS.

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Spinal Cord InjuryRegenerative MedicineResearch Methodology & Design

Identification of key genes involved in recovery from spinal cord injury in adult zebrafish

Neural Regen Res, 2022 • June 1, 2022

This study aimed to identify key genes and pathways involved in axon regeneration after spinal cord injury (SCI) in zebrafish to provide new targets for mammalian SCI treatment. RNA sequencing data an...

KEY FINDING: Zebrafish spontaneously recovered 44% of their swimming ability within the subacute phase (2 weeks) after spinal cord injury.

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OncologyResearch Methodology & Design

Toll-7 promotes tumour growth and invasion in Drosophila

Cell Proliferation, 2022 • January 2, 2022

This study identifies Toll-7 as a proto-oncogene in Drosophila that promotes tumor growth and invasion. Toll-7 activates Egr-JNK and EGFR-Ras signaling pathways, leading to cell proliferation, tissue ...

KEY FINDING: Loss of Toll-7 suppresses tumor growth and invasion in a Drosophila model of RasV12/lgl−/−-induced tumors.

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Regenerative MedicineGeneticsResearch Methodology & Design

Hippo-Yap/Taz signalling in zebrafish regeneration

npj Regenerative Medicine, 2022 • January 1, 2022

This review summarizes the role of the Hippo pathway in zebrafish regeneration, noting that Yap1/Taz signaling often enhances regeneration through promoting cell proliferation, progenitor cell dediffer...

KEY FINDING: Hippo pathway effectors Yap/Taz facilitate zebrafish regeneration, suggesting therapeutic potential in promoting YAP/TAZ signaling to enhance mammalian regeneration.

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Regenerative MedicineNeurologyResearch Methodology & Design

Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing

STAR Protocols, 2022 • March 18, 2022

Zebrafish regenerate their spinal cord after injury, both at larval and adult stages. Larval zebrafish have emerged as a powerful model system to study spinal cord injury and regeneration due to their h...

KEY FINDING: The protocol details two methods for spinal cord transection: incision and perforation lesion, both yielding comparable regeneration kinetics with reproducibility, high throughput, and low mortality.

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ImmunologyBioinformaticsResearch Methodology & Design

Sample Buffer Containing Guanidine-Hydrochloride Combines Biological Safety and RNA Preservation for SARS-CoV-2 Molecular Diagnostics

Diagnostics, 2022 • May 10, 2022

The study addresses the need for a sample storage buffer (SSB) that inactivates SARS-CoV-2 while preserving viral RNA for reliable diagnostics and sequencing. The developed guanidine-hydrochloride-bas...

KEY FINDING: The guanidine-hydrochloride-based storage buffer (SSB-4M) is compatible with commercially available and in-house GuHCl-based RNA extraction methods.

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Regenerative MedicineNeurologyResearch Methodology & Design

Uncovering the spectrum of adult zebrafish neural stem cell cycle regulators

Front. Cell Dev. Biol., 2022 • June 29, 2022

This review examines the factors that regulate adult neural stem and progenitor cell (aNSPC) cell cycle dynamics in teleosts, particularly focusing on studies using the zebrafish model. It highlights h...

KEY FINDING: Social interactions, stress, hormones, diet, and sensory stimuli significantly influence aNSPC activity in the zebrafish CNS.

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