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  4. Electrospun fibers for drug delivery after spinal cord injury and the effects of drug incorporation on fiber properties

Electrospun fibers for drug delivery after spinal cord injury and the effects of drug incorporation on fiber properties

Cells Tissues Organs, 2016 · DOI: 10.1159/000446621 · Published: January 1, 2016

Spinal Cord InjuryPharmacologyBiomedical

Simple Explanation

Spinal cord injuries (SCI) lack a cure, with current treatments showing limited functional recovery. Biomaterials, especially electrospun fibers, are being explored to guide nerve regeneration and deliver drugs to the injury site. The study investigates how incorporating riluzole or neurotrophin-3 (NT-3) affects the physical properties of electrospun fibers made from poly-L-lactic acid (PLLA). The experiment showed that both drugs were successfully incorporated into the fibers. However, the inclusion of the drugs altered the fibers' physical characteristics. Specifically, fiber diameter decreased with the inclusion of the drug, and the decreased diameter was correlated with a decrease in fiber alignment. These changes in fiber properties are important because they can affect how cells and axons respond to the scaffold, potentially impacting the success of nerve regeneration. The authors discuss considerations for incorporating therapeutic drugs without changing the physical properties of the fibers.

Study Duration
Not specified
Participants
Not specified
Evidence Level
Not specified

Key Findings

  • 1
    Riluzole and NT-3 were successfully incorporated into electrospun PLLA fibers.
  • 2
    Inclusion of either riluzole or NT-3 in electrospun fibers caused a reduction in fiber diameter.
  • 3
    Inclusion of either riluzole or NT-3 in electrospun fibers interfered with fiber alignment.

Research Summary

This paper reviews the use of electrospun fibers for drug delivery in spinal cord injury (SCI) treatment, highlighting their potential to guide axonal regeneration and deliver therapeutic drugs locally. The experimental section focuses on incorporating riluzole and neurotrophin-3 (NT-3) into poly-L-lactic acid (PLLA) electrospun fibers, examining the impact of drug concentration on fiber alignment and diameter. The study concludes that while drug incorporation is successful, it alters fiber physical properties, specifically decreasing fiber diameter and alignment. These alterations can affect cellular and axonal responses, necessitating careful optimization of drug-eluting scaffolds.

Practical Implications

Optimizing Drug-Eluting Scaffolds

Researchers need to carefully optimize drug-eluting scaffolds to maintain desired fiber characteristics for effective nerve regeneration.

Tailoring Fiber Properties

Fiber diameter and alignment can be tailored to influence cellular responses, which is crucial for promoting axonal regeneration.

Selecting Appropriate Drug

The choice of drug (small molecule vs. large molecule) and its concentration have different effects on fiber morphology, requiring careful consideration.

Study Limitations

  • 1
    Study lacks in-vivo experimentation
  • 2
    Solubility limitations made comparisons between riluzole and NT-3 difficult
  • 3
    Focused only on diameter and alignment, neglecting other fiber properties.

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