Tissue-Engineered Regeneration of Completely Transected Spinal Cord Using Induced Neural Stem Cells and Gelatin-Electrospun Poly (Lactide-Co-Glycolide)/Polyethylene Glycol Scaffolds

This study explores a new approach to treating spinal cord injuries using tissue engineering. The method involves using induced neural stem cells (iNSCs) and a special scaffold to help regenerate damaged spinal cord tissue. Mouse embryonic fibroblasts were reprogrammed into neural stem cells (iNSCs). These cells, along with a three-dimensional (3D) scaffold made of PLGA-PEG nanofibers, were used to promote cell growth and adhesion. The iNSC-seeded scaffolds were transplanted into rats with completely transected spinal cords. The results showed that the iNSCs survived, differentiated into neurons and glial cells, and contributed to functional recovery of the spinal cord.

• 1
  Induced neural stem cells (iNSCs) can be generated from mouse embryonic fibroblasts (MEFs) through direct reprogramming.
• 2
  PLGA-PEG nanofiber scaffolds support iNSC adhesion, growth, survival, and differentiation in vitro and in vivo.
• 3
  Transplantation of iNSC-seeded PLGA-PEG scaffolds into transected rat spinal cords promotes functional recovery and reduces cavity formation.