Research of diclofenac sodium-loaded gelatin scaffold with anti-inflammatory activity for promoting in vivo cartilage regeneration

Chinese Journal of Reparative and Reconstructive Surgery, 2023 · DOI: 10.7507/1002-1892.202207114 · Published: January 1, 2023

Pharmacology Regenerative Medicine Biomedical

Simple Explanation

The study aimed to create a scaffold that releases diclofenac sodium, an anti-inflammatory drug, to help reduce inflammation and promote cartilage regeneration after implantation. The scaffold was made from gelatin and diclofenac sodium using a freeze-drying method, and its properties were tested in the lab and in rabbits. The results showed that the scaffold reduced inflammation and supported cartilage regeneration in rabbits, suggesting it could be a useful tool for cartilage repair.

Study Duration

4 Weeks

Participants

New Zealand white rabbits and RAW264.7 macrophages

Evidence Level

Not specified

Key Findings

1
The diclofenac sodium-loaded gelatin scaffold significantly reduced the expression of IL-1β and TNF-α, indicating its anti-inflammatory properties.
2
In vitro experiments showed that the scaffold supported chondrocyte survival and proliferation, demonstrating good cell compatibility.
3
In vivo experiments demonstrated that the diclofenac sodium-loaded scaffold promoted cartilage regeneration and reduced inflammation-related gene expression (CD3 and CD68) in rabbits.

Research Summary

This study developed a diclofenac sodium-loaded gelatin scaffold with anti-inflammatory activity to promote cartilage regeneration in vivo. The scaffold exhibited suitable pore size, porosity, and cytocompatibility, and demonstrated satisfactory anti-inflammatory ability in vitro and in vivo. The diclofenac sodium-loaded gelatin scaffold provides a reliable scheme for alleviating the inflammatory reaction of regenerated cartilage tissue after in vivo implantation and promoting cartilage regeneration in vivo.

Practical Implications

Enhanced Cartilage Repair

The scaffold can be used to create an anti-inflammatory microenvironment to improve cartilage regeneration.

Reduced Inflammation

The diclofenac sodium-loaded scaffold could reduce inflammation, improving cartilage repair outcomes.

Clinical Applications

This approach has potential for clinical translation in cartilage defect repair.

Study Limitations

1
The study needs further investigation in larger animals (e.g., sheep, dogs, pigs) to confirm the anti-inflammatory effects.
2
The optimal dosage of diclofenac sodium in the scaffold requires further optimization.
3
Long-term stability and maintenance of the regenerated cartilage tissue in vivo need to be assessed.

Your Feedback

Was this summary helpful?

Back to Pharmacology