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  4. PLG bridge implantation in chronic SCI promotes axonal elongation and myelination

PLG bridge implantation in chronic SCI promotes axonal elongation and myelination

ACS Biomater Sci Eng., 2019 · DOI: 10.1021/acsbiomaterials.9b01012 · Published: December 9, 2019

Spinal Cord InjuryBiomedical

Simple Explanation

This study investigates a potential treatment for chronic spinal cord injury (SCI) using a bridge made of poly(lactide-co-glycolide) (PLG). The PLG bridge is surgically implanted into the injured spinal cord to promote tissue regeneration. The goals of this investigation were to create a reproducible chronic injury model and to show PLG bridges are a growth permissive substrate for axonal elongation and tissue reformation after chronic spinal cord injury. The study found that the PLG bridge supported axon growth and myelination in the chronic SCI model, suggesting it could be a useful platform for future therapies. The process of bridge implantation did not significantly decrease the post injury function.

Study Duration
6 months
Participants
42 C57BL/6 mice
Evidence Level
Not specified

Key Findings

  • 1
    PLG bridges implanted into chronic SCI promote axonal regeneration into the injury site.
  • 2
    Chronically regenerated axons within the PLG bridges are myelinated, primarily by Schwann cells.
  • 3
    Bridge implantation does not significantly decrease functional outcomes following chronic SCI.

Research Summary

This study investigated regeneration using a poly(lactide-co-glycolide) (PLG) multi-channel bridge implanted into a chronic SCI following surgical resection of necrotic tissue. These data demonstrate that bridge implantation into chronic SCI supports regeneration and provides a platform to investigate strategies to buttress and expand regeneration of neural tissue at chronic time points. The chronic injury model was based on our previous acute penetrating lateral hemisection model and other models of chronic SCI.

Practical Implications

Therapeutic Potential

PLG bridges may serve as a promising therapeutic intervention for chronic SCI by promoting axonal regeneration and myelination.

Platform for Further Research

The PLG bridge provides a platform to investigate strategies to buttress and expand regeneration of neural tissue at chronic time points, such as delivering bioactive agents to promote neuron intrinsic growth and chemoattraction.

Clinical Translation

The chronic SCI model developed in this study follows emerging clinical practices and the pathophysiology of most patients, facilitating translation of findings to human studies.

Study Limitations

  • 1
    This model does not fully recapitulate human SCI in some notable areas.
  • 2
    The loss of functional recovery from tissue resection is a significant limitation in chronic injury treatment.
  • 3
    Few studies have noted functional improvements in chronic spinal cord even with the additions of cells or bioactive factors.

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