Spinal Cord Research Help
AboutCategoriesLatest ResearchContact
Subscribe
Spinal Cord Research Help

Making Spinal Cord Injury (SCI) Research Accessible to Everyone. Simplified summaries of the latest research, designed for patients, caregivers and anybody who's interested.

Quick Links

  • Home
  • About
  • Categories
  • Latest Research
  • Disclaimer

Contact

  • Contact Us
© 2025 Spinal Cord Research Help

All rights reserved.

  1. Home
  2. Research
  3. Spinal Cord Injury
  4. Custom-engineered hydrogels for delivery of human iPSC-derived neurons into the injured cervical spinal cord

Custom-engineered hydrogels for delivery of human iPSC-derived neurons into the injured cervical spinal cord

Biomaterials, 2024 · DOI: 10.1016/j.biomaterials.2023.122400 · Published: March 1, 2024

Spinal Cord InjuryRegenerative MedicineBiomedical

Simple Explanation

This research explores a new approach to treating cervical spinal cord injuries by combining a specially designed injectable hydrogel with human stem cell-derived neurons. The hydrogel is designed to mimic the natural environment of cells and can be customized to promote cell adhesion and control the gel's mechanical properties. In laboratory tests, the hydrogel was found to support the survival and growth of the transplanted neurons. When injected into rats with cervical spinal cord injuries, the hydrogel biodegraded without causing adverse reactions, improved the consistency of cell transplantation, and aided the integration of cells into the host tissue. The results showed significant improvement in sensorimotor function in the rats that received the combined therapy, suggesting that this approach could be a promising treatment for cervical spinal cord injuries.

Study Duration
6 Weeks
Participants
Female adult athymic RNU rats
Evidence Level
Not specified

Key Findings

  • 1
    SHIELD with the RGDS ligand demonstrated the highest cell viability and the most robust neurite outgrowth in vitro.
  • 2
    Delivery in SHIELD resulted in more reproducible transplantation of viable hiPSC-DCNs.
  • 3
    Animals treated with hiPSC-DCNs delivered in SHIELD exhibited significant improvement in behavioral function

Research Summary

This study presents the design of a delivery vehicle customized for transplantation of human cortical projection neurons into the injured cervical spinal cord, utilizing a family of SHIELD gels with different cell-adhesive ligands and stiffness. The optimized SHIELD variant with the RGDS binding ligand and the most compliant stiffness was identified as most appropriate for hiPSC-DCN culture in vitro, demonstrating higher neuron viability compared to cells delivered in saline. In a rat model of cervical spinal cord injury, SHIELD improved transplant consistency, promoted neuritic elongation, and resulted in significant improvement in behavioral function compared to cell transplantation in saline or injection of SHIELD alone.

Practical Implications

Improved Cell Transplantation

SHIELD hydrogels offer a more reliable method for transplanting sensitive cells like iPSC-derived neurons, potentially increasing the success rate of cell-based therapies for spinal cord injuries.

Customizable Therapy Design

The modular design of SHIELD allows for tailoring the hydrogel's properties to match specific cell types and injury conditions, enabling more effective and personalized treatment strategies.

Enhanced Functional Recovery

The study demonstrates that SHIELD-mediated delivery of iPSC-derived neurons can lead to significant improvements in sensorimotor function after cervical spinal cord injury, offering hope for improved patient outcomes.

Study Limitations

  • 1
    [object Object]
  • 2
    [object Object]
  • 3
    [object Object]

Your Feedback

Was this summary helpful?

Back to Spinal Cord Injury