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  4. Weight-Bearing Locomotion in the Developing Opossum, Monodelphis domestica following Spinal Transection: Remodeling of Neuronal Circuits Caudal to Lesion

Weight-Bearing Locomotion in the Developing Opossum, Monodelphis domestica following Spinal Transection: Remodeling of Neuronal Circuits Caudal to Lesion

PLoS ONE, 2013 · DOI: 10.1371/journal.pone.0071181 · Published: August 12, 2013

Spinal Cord InjuryNeurologyGenetics

Simple Explanation

This study investigates how the nervous system repairs itself after spinal injuries early in development. Researchers used marsupial opossums because their young are born very immature. Opossum pups had their spinal cords completely cut at either 7 days old or 28 days old, and then were allowed to grow into adults. The researchers then looked at how well they could move. The study found that opossums injured at 7 days old could regain near-normal walking ability, but those injured at 28 days old could only perform basic stepping movements. This suggests that the age at which the injury occurs greatly impacts the nervous system's ability to recover.

Study Duration
Not specified
Participants
27 opossums (Monodelphis domestica): Control (n = 11), P7-injured (n = 7), and P28-injured (n = 9)
Evidence Level
Not specified

Key Findings

  • 1
    P7-injured animals regrew supraspinal and propriospinal axons through the injury site and recovered near-normal coordinated overground locomotion with altered gait characteristics.
  • 2
    P28-injured animals showed no axonal regrowth through the injury site, yet they could perform weight-supporting hindlimb stepping overground and on the treadmill.
  • 3
    Swimming tests indicated that P7-injured animals used their hindlimbs, suggesting functional connections from axons that grew across the lesion, while P28-injured animals only used forelimbs, indicating reflex-dependent hindlimb movements.

Research Summary

The study investigated spinal cord injuries in developing opossums at postnatal days 7 and 28 to understand neuronal circuit remodeling and locomotor recovery. P7-injured opossums showed axonal regrowth and near-normal locomotion, while P28-injured opossums lacked axonal regrowth but exhibited weight-supporting stepping, suggesting different recovery mechanisms. The findings indicate that the isolated segment of the spinal cord retains some capability of rhythmic movement, although the mechanisms involved in weight-bearing locomotion are distinct.

Practical Implications

Understanding Spinal Cord Plasticity

The study enhances understanding of spinal cord plasticity and spontaneous recovery mechanisms in early development, particularly the differences between injuries at different developmental stages.

Developing Targeted Therapies

The identification of propriospinal circuit remodeling in the absence of supraspinal input could lead to targeted therapies that enhance local spinal cord circuitry function after injury.

Refining Animal Models

The research highlights the importance of choosing appropriate animal models (e.g., bipedal models) for spinal cord injury studies to better translate findings to human patients.

Study Limitations

  • 1
    Inability to perform complete spinal transections in control, adult opossums long enough to study locomotion post-injury.
  • 2
    The Monodelphis genome is not well annotated and no commercially available molecular tools are available.
  • 3
    Lack of commercially available antibodies to antigens from other animal species that cross-react with Monodelphis tissue.

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