Upregulation of mitotic bookmarking factors during enhanced proliferation of human stromal cells in human platelet lysate

J Transl Med, 2019 · DOI: https://doi.org/10.1186/s12967-019-02183-0 · Published: December 20, 2019

Regenerative Medicine Genetics Research Methodology & Design

Simple Explanation

The study compares different preparations of human platelet lysate (HPL) to fetal bovine serum (FBS) for growing human stromal cells. HPL is explored as a xeno-free alternative to FBS, which carries risks of animal pathogen transmission and ethical concerns. The research investigates how these different culture conditions affect the cells' ability to grow, maintain their characteristics, and express specific genes that help them quickly re-enter the cell cycle.

Study Duration

Not specified

Participants

Stromal cells isolated from bone marrow (BM), white adipose tissue (WAT) and umbilical cord (UC)

Evidence Level

Not specified

Key Findings

1
pHPL-supplemented media supported proliferation of WAT- and UC-derived stromal cells significantly better than FBS.
2
mRNA expression of mitotic bookmarking factors cMYC and KLF4 was significantly enhanced in stromal cells cultured in pHPL compared to FBS.
3
SOX2 mRNA expression of all stromal cell types was increased in all pHPL culture conditions.

Research Summary

The study systematically compares pooled HPL-based medium preparations (pHPL) and two differentially fibrinogen-depleted variants and FBS with respect to biochemical composition. Cell proliferation was significantly enhanced in pHPL-based media independent of fibrinogen and heparin, compared to FBS supplementation. mRNA expression levels of the mitotic bookmarking factors SOX2, cMYC and KLF4 were significantly elevated in stromal cells cultured in pHPL-based media in comparison to FBS-supplemented medium.

Practical Implications

Standardization of Culture Media

The results support better characterization and standardization of humanized culture media for stromal cell-based medicinal products.

Enhanced Cell Proliferation

The use of pHPL can significantly enhance cell proliferation compared to FBS, which is crucial for cell-based therapies.

Mitotic Bookmarking Factors

The enhanced expression of mitotic bookmarking factors in pHPL-expanded cells suggests a mechanism for faster cell cycle re-entry, potentially improving therapeutic efficacy.

Study Limitations

1
Manufacturing protocols for stromal cell therapeutics are highly variable.
2
Divergent observations further highlight the need for an extended standardization of pHPL as raw material.
3
The significance of our observation for in vivo function of stromal cell therapeutics has to be further investigated.

Your Feedback

Was this summary helpful?

Back to Regenerative Medicine