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  4. Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord

Transplantation of Adult Rat Schwann Cells into the Injured Spinal Cord

Methods Mol Biol, 2018 · DOI: 10.1007/978-1-4939-7649-2_28 · Published: January 1, 2018

Spinal Cord InjuryRegenerative MedicineNeurology

Simple Explanation

Adult Schwann cells (SCs) can be transplanted into the injured spinal cord to provide a supportive environment for nerve fiber growth and to help insulate and protect these fibers with myelin. Transplanting peripheral nerves or purified SCs into the injured spinal cord can encourage nerve fiber regeneration and myelination by the SCs. The effects of SC transplants alone on SCI repair are modest. Significant experimental effort has focused on combining SC transplants with additional strategies to enhance their efficacy for SCI repair.

Study Duration
Not specified
Participants
Rats
Evidence Level
Not specified

Key Findings

  • 1
    Schwann cell transplants can promote axonal regeneration in the injured spinal cord.
  • 2
    Purified Schwann cells can remyelinate and ensheath axons within the injured spinal cord.
  • 3
    Axons within Schwann cell grafts are able to conduct action potentials.

Research Summary

The article describes the collection and transplantation of adult rat primary SCs into the injured spinal cord, including tissue culture procedures and surgical methods for spinal cord injury induction and cell injection. Schwann cells can be used to promote axonal regeneration and remyelination in the injured spinal cord, offering a potential therapeutic approach for spinal cord injury repair. The described methods provide a foundation for developing combination strategies to enhance the utility of Schwann cell transplants for spinal cord injury repair.

Practical Implications

Therapeutic Potential

Schwann cell transplantation offers a potential therapeutic strategy for promoting axonal regeneration and functional recovery after spinal cord injury.

Combination Therapies

Combining Schwann cell transplantation with other therapeutic interventions may enhance the efficacy of spinal cord injury repair.

Preclinical Model

The described methods provide a valuable preclinical model for assessing the ability of Schwann cells to promote spinal cord repair.

Study Limitations

  • 1
    Efficacy of SC transplants alone on SCI repair are modest.
  • 2
    In rats, but not mice, contusion injuries produce a cystic cavity similar to human SCI.
  • 3
    Transplantation can be performed any time following injury.

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