in vivo, 2022 · DOI: 10.21873/invivo.12929 · Published: July 12, 2022
This study explores methods for culturing olfactory ensheathing cells (OECs) using materials suitable for creating advanced cell therapies for humans. The focus is on spinal cord regeneration. The research compares different collagen types for creating scaffolds to support OECs during surgical procedures. It also examines optimal conditions, like surface modifications and serum concentrations, for growing these cells. The findings suggest that OECs can be successfully cultured using human-grade reagents, leading to a functional advanced therapy medicinal product for potential use in spinal cord injury treatment.
The study provides insights into optimizing OEC culture methods using certified materials, facilitating the production of advanced cell therapy products for spinal cord injury treatment.
The finding that acid-solubilized collagen is superior for scaffold preparation can improve the handling and transplantation of OECs during surgical procedures.
The identification of fibronectin as a preferred surface modification and the determination of the optimal FBS concentration can enhance OEC yield and quality for therapeutic applications.