The superiority of conditioned medium derived from rapidly expanded mesenchymal stem cells for neural repair

Stem Cell Research & Therapy, 2019 · DOI: https://doi.org/10.1186/s13287-019-1491-7 · Published: December 1, 2019

Spinal Cord Injury Regenerative Medicine

Simple Explanation

Spinal cord injury is a severe neurological condition, and mesenchymal stem cells (MSCs) show promise for regenerative medicine. This study investigates the effect of hMSC-conditioned medium supplemented with bFGF, EGF, and patient plasma, named neural regeneration laboratory medium (NRLM), on SCI in vitro and in vivo. Compared to standard culture medium, NRLM-CM had superior inflammation reduction and neurite regeneration effects in vitro and improved functional restoration in SCI rats in vivo.

Study Duration

8 weeks

Participants

30 patients with cervical, thoracic, or lumbar diseases undergoing spinal fusion surgery and adult female Sprague-Dawley rats

Evidence Level

Level 2: In vitro and In vivo studies

Key Findings

1
NRLM-CM had superior inflammation reduction and neurite regeneration effects in vitro compared to standard culture medium.
2
NRLM-CM improved functional restoration in SCI rats in vivo compared to standard culture medium.
3
NRLM-MSCs proliferated faster than standard culture medium MSCs regardless of the donor's age.

Research Summary

This study introduces NRLM, a culture medium for clinical hMSC expansion, which enhances yields and therapeutic effects for SCI repair. NRLM was shown to increase the fold expansion of MSCs and markedly elevated the growth rate of MSCs from older donors. The study results suggest that the observed superiority of NRLM-CM over MSCGM-CM may result from secreted factors like adiponectin, ANG, and HGF.

Practical Implications

Clinical Applications

The NRLM culture system can be used for the large-scale production of functionally competent MSCs for clinical applications in SCI treatment.

Therapeutic Potential

The superior neural repair function of conditioned medium derived from NRLM-expanded MSCs highlights its therapeutic potential.

Enhanced MSC Expansion

NRLM provides a means to rapidly expand MSCs, even from older donors, which can reduce manufacturing time and accelerate MSC production.

The superiority of conditioned medium derived from rapidly expanded mesenchymal stem cells for neural repair

Simple Explanation

Key Findings

Research Summary

Practical Implications

Clinical Applications

Therapeutic Potential

Enhanced MSC Expansion

Study Limitations

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Was this summary helpful?

The superiority of conditioned medium derived from rapidly expanded mesenchymal stem cells for neural repair

Simple Explanation

Key Findings

Research Summary

Practical Implications

Clinical Applications

Therapeutic Potential

Enhanced MSC Expansion

Study Limitations

Your Feedback

Was this summary helpful?