Strategies for neurotrophin-3 and chondroitinase ABC release from freeze-cast chitosan–alginate nerve-guidance scaffolds

This study explores the use of special scaffolds to help nerves regrow after spinal cord injuries. These scaffolds are designed to release helpful proteins, specifically neurotrophin-3 (NT-3) and chondroitinase ABC (chABC), which can encourage nerve regeneration. The scaffolds are made from chitosan and alginate, materials that are compatible with the body. The researchers tested how well these scaffolds could release NT-3 and chABC over time and whether these released proteins could still stimulate nerve growth. The results showed that the scaffolds could release both NT-3 and chABC for several weeks, and these proteins remained active, promoting nerve growth in lab tests. This suggests that these scaffolds could be a promising tool for treating spinal cord injuries.

• 1
  NT-3 was released from the C–A scaffolds for 8 weeks in vitro, while chABC was released for up to 7 weeks.
• 2
  Low total percentages of protein released from the scaffolds over this time period were attributed to limitation of diffusion by the interpenetrating polymer network matrix of the scaffold walls.
• 3
  NT-3 and chABC released from the scaffolds retained bioactivity, as determined by a neurite outgrowth assay, and the promotion of neurite growth across an inhibitory barrier of chondroitin sulphate proteoglycans.