Spinal Cord Injury Affects Gene Expression of Transmembrane Proteins in Tissue and Release of Extracellular Vesicle in Blood: In Silico and In Vivo Analysis

This study investigates how spinal cord injury (SCI) affects genes that control transmembrane proteins, which are crucial for cell communication. By analyzing gene expression data and conducting experiments on rats with SCI, the researchers identified specific transmembrane proteins that change expression levels after injury. The research also explores the role of extracellular vesicles (EVs), tiny particles released by cells, in the context of SCI. EVs can carry signals and influence the function of other cells, potentially affecting the progression or recovery from spinal cord injuries. The study examines how SCI impacts the release and composition of EVs in the blood. By combining computational analysis with experimental validation, the study aims to provide insights into the molecular mechanisms underlying SCI and identify potential targets for therapeutic interventions. The findings suggest that transmembrane proteins and EVs play significant roles in the response to SCI and could be harnessed to improve recovery strategies.

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  Significant upregulation of Grm1, Nrg1, CD63, Enpp3, and Cxcr4 was observed between the acute and control groups at the RNA level.
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  Downregulation in Enpp3 was found between acute and subacute groups at the RNA level.
• 3
  CD9, an EV marker, showed significant expression differences between acute and control groups in plasma-derived EVs.