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  4. Silencing long ascending propriospinal neurons after spinal cord injury improves hindlimb stepping in the adult rat

Silencing long ascending propriospinal neurons after spinal cord injury improves hindlimb stepping in the adult rat

eLife, 2021 · DOI: https://doi.org/10.7554/eLife.70058 · Published: December 2, 2021

Spinal Cord InjuryNeurologyRehabilitation

Simple Explanation

This study investigates the role of long ascending propriospinal neurons (LAPNs) in locomotor recovery after spinal cord injury (SCI) in rats. LAPNs are spinal cord interneurons that connect the lumbar and cervical enlargements, and they are thought to play a role in coordinating limb movements during locomotion. The researchers conditionally silenced LAPNs after SCI and found that, contrary to their hypothesis, silencing LAPNs improved locomotor function. This included better paw placement order and timing, fewer dorsal steps, restored left-right hindlimb coordination, and normalized gait spatiotemporal features. These unexpected findings suggest that spared LAPNs post-SCI may interfere with hindlimb stepping and that silencing them may remove excess 'noise' within the locomotor system, allowing intrinsic lumbar circuitry to function independently.

Study Duration
Not specified
Participants
10 adult female Sprague-Dawley rats
Evidence Level
Not specified

Key Findings

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    Silencing spared LAPNs post-SCI improved locomotor function, including paw placement order and timing.
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    Silencing spared LAPNs post-SCI led to a decrease in the number of dorsal steps.
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    Silencing restored left-right hindlimb coordination and normalized spatiotemporal features of gait such as stance and swing time.

Research Summary

The study investigates the role of long ascending propriospinal neurons (LAPNs) in locomotor recovery after spinal cord injury (SCI) in rats, conditionally silencing LAPNs after SCI to observe the effects on locomotion. Unexpectedly, silencing spared LAPNs post-SCI improved various aspects of locomotor function, including paw placement order and timing, a decrease in dorsal steps, restored left-right hindlimb coordination, and normalized gait spatiotemporal features. The findings suggest that spared LAPNs may interfere with hindlimb stepping after thoracic SCI and that silencing them may remove excess 'noise' within the locomotor system, allowing intrinsic lumbar circuitry to function independently.

Practical Implications

Therapeutic Strategies

These findings have implications for the development of neuronal- and axonal-protective therapeutic strategies, suggesting that anatomical sparing may not always result in expected benefits.

Clinical Study Implementation

The results may influence the clinical study and implementation of neuromodulation strategies, such as epidural stimulation, by considering the potential maladaptive roles of spared pathways like LAPNs.

Understanding Locomotor Recovery

The study contributes to a better understanding of the complex mechanisms underlying locomotor recovery after SCI, highlighting the potential for spared neural circuits to have detrimental effects on function.

Study Limitations

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