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  4. Restorative Effects of Human Neural Stem Cell Grafts to the Primate Spinal Cord

Restorative Effects of Human Neural Stem Cell Grafts to the Primate Spinal Cord

Nat Med, 2018 · DOI: 10.1038/nm.4502 · Published: May 1, 2018

Spinal Cord InjuryRegenerative MedicineNeurology

Simple Explanation

Human spinal cord-derived neural progenitor cells were grafted into sites of cervical spinal cord injury in rhesus monkeys to test their restorative effects. The grafts survived for at least 9 months, expressed both neuronal and glial markers, and facilitated the regeneration of monkey axons into the grafts. Hundreds of thousands of human axons extended from the grafts into the monkey's white matter, forming synapses in the gray matter and leading to improved forelimb function after a few months.

Study Duration
9 Months
Participants
9 adult male rhesus monkeys
Evidence Level
Not specified

Key Findings

  • 1
    Human neural stem cells can be successfully engrafted to fill the large lesion cavity of a non-human primate spinal cord.
  • 2
    Human grafts exhibit a prolonged time period of maturation, with functional effects evident by 3–4 months post-grafting.
  • 3
    Primate corticospinal axons also regenerated into human neural stem cells grafted to lesion sites.

Research Summary

This study investigated the potential of human neural stem cell grafts to restore function after spinal cord injury in rhesus monkeys. The results showed that the grafts survived, matured, and integrated with the host spinal cord, leading to the regeneration of axons and improved forelimb function. The findings support the feasibility of using neural stem cell grafting as a therapeutic approach for spinal cord injury in humans, with the objective of reconstituting both a neuronal and glial environment at the injury site.

Practical Implications

Clinical Translation

The study provides preclinical evidence supporting the translation of human neural stem cell grafting to humans with spinal cord injury.

Therapeutic Target

The findings suggest that neural stem cell grafting can reconstitute both a neuronal and glial milieu in the site of spinal cord injury, providing a potential therapeutic target.

Overcoming Regeneration Barriers

The study demonstrates that neural stem cell therapy can circumvent the axon-growth-inhibitory nature of adult myelin, a major challenge in the regeneration field.

Study Limitations

  • 1
    The study did not contain a lesioned control group in which no attempt to graft was made.
  • 2
    Monkeys with poor graft survival did not survive as long as monkeys with surviving grafts.
  • 3
    It is possible that more prolonged observation periods could result in yet greater recovery.

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