Reducing neuroinflammation by delivery of IL-10 encoding lentivirus from multiple-channel bridges

The spinal cord's inability to regenerate after injury is primarily due to an inflammatory response that hinders growth, leading to further damage and cell death. The study focuses on reducing this inflammation by promoting a shift in immune cells towards anti-inflammatory types. The research uses multiple-channel bridges to deliver a virus encoding IL-10, an anti-inflammatory factor, directly to the injured area. This approach aims to locally modify the inflammatory response and create a more supportive environment for nerve regeneration. The study found that localized delivery of IL-10 modulated the immune response, reducing neutrophil infiltration and skewing macrophages toward an anti-inflammatory phenotype, ultimately improving motor function in the injured mice.

• 1
  IL-10 gene delivery from bridges reduces neutrophil infiltration at both day 7 and day 28 post-injury, suggesting a sustained anti-inflammatory effect.
• 2
  While IL-10 delivery did not affect the overall number of macrophages, it shifted the macrophage population toward an anti-inflammatory M2 phenotype and altered macrophage morphology.
• 3
  Delivery of IL-10 resulted in improved motor function in the mice, indicating reduced secondary damage and increased tissue sparing following spinal cord injury.