Necroptosis pathway emerged as potential diagnosis markers in spinal cord injury

J Cell Mol Med, 2024 · DOI: 10.1111/jcmm.18219 · Published: February 22, 2024

Spinal Cord Injury Genetics Bioinformatics

Simple Explanation

This study aimed to identify genes related to necroptosis that are differentially expressed in spinal cord injury (SCI) to find potential targets for therapy and prognosis. The researchers analyzed three SCI datasets and identified necroptosis-related genes (NRGs). They then found the overlap between differentially expressed genes (DEGs) and NRGs to identify NRDEGs. Using various bioinformatics tools, they predicted interactions between key genes and other molecules, analyzed immune cell infiltration, and verified their findings in an animal model of SCI.

Study Duration

Not specified

Participants

Three SCI-related datasets (GSE151371, GSE5296 and GSE47681) and animal model of SCI (n = 3/group)

Evidence Level

Level 5: Bioinformatics analysis and animal model study

Key Findings

1
Combined analysis identified 15 co-expressed DEGs and NRGs, highlighting pathways associated with necroptosis and apoptosis.
2
Hub gene expression analysis showed that the expression of CHMP7 and FADD was highly accurate in diagnosing SCI.
3
CHMP7 and FADD were identified as potential therapeutic targets for SCI.

Research Summary

This research focused on identifying necroptosis-related differentially expressed genes (NRDEGs) in spinal cord injury (SCI) to find potential therapeutic and prognostic target genes. The study identified 15 DEGs associated with necroptosis after SCI, observing significant expression differences, with six genes upregulated and nine downregulated. The top 10 hub genes could potentially serve as diagnostic biomarkers for SCI-induced necroptosis and provide a foundation and potential therapeutic target for SCI-induced necrotizing apoptosis.

Practical Implications

Diagnostic Biomarkers

The identified hub genes can serve as diagnostic biomarkers for SCI-induced necroptosis.

Therapeutic Targets

Upregulation of FADD and downregulation of CHMP7 following SCI may be indicative of necroptosis, providing potential therapeutic targets.

Immunological Insights

The study provides insights into the correlation between gene expression and immune cell infiltration, suggesting potential targets to modulate the immune response after SCI.

Study Limitations

1
Primary data was derived from online databases, and a small sample size was adopted.
2
Identified hub genes should be validated in SCI samples through cell culture and animal experiments.
3
The work lacked further mechanistic validation.

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