MiR‑155‑5p Aggravated Astrocyte Activation and Glial Scarring in a Spinal Cord Injury Model by Inhibiting Ndfip1 Expression and PTEN Nuclear Translocation

This study investigates the role of miR-155-5p in astrocyte activation and glial scarring following spinal cord injury (SCI). The researchers found that miR-155-5p overexpression promotes astrocyte proliferation and inhibits cell apoptosis in vitro. The study also found that miR-155-5p inhibits nuclear PTEN expression by targeting Ndfip1. Overexpression of Ndfip1 reversed astrocyte activation induced by miR-155-5p mimic and abolished the inhibition effect of miR-155-5p mimic on PTEN nuclear translocation. In vivo experiments showed that miR-155-5p silencing improved locomotor function in SCI rats and reduced astrocyte activation and glial scar formation. These findings suggest that miR-155-5p could be a potential therapeutic target for SCI.

• 1
  MiR-155-5p overexpression promotes astrocyte proliferation, invasion, and inhibits apoptosis in vitro, suggesting its role in astrocyte activation.
• 2
  MiR-155-5p inhibits nuclear PTEN expression by directly targeting Ndfip1, a protein involved in ubiquitination and protein degradation.
• 3
  Silencing miR-155-5p in vivo improves locomotor function in SCI rats, reduces astrocyte activation, and decreases glial scar formation, indicating its potential as a therapeutic target.