miR-434-3p and DNA hypomethylation co-regulate eIF5A1 to increase AChRs and to improve plasticity in SCT rat skeletal muscle

This study investigates the role of microRNAs (miRNAs) in modulating acetylcholine receptors (AChRs) after spinal cord transection (SCT) in rats. It identifies miR-434-3p as a key regulator of eIF5A1 expression, which in turn influences AChRs expression and locomotor function. The research demonstrates that miR-434-3p negatively regulates eIF5A1, and its depletion in SCT rat skeletal muscle leads to increased AChRs numbers and improved function scores. Conversely, overexpression of miR-434-3p reduces AChRs expression and impairs motor function recovery. Additionally, the study reveals that DNA demethylation also up-regulates eIF5A1 after SCT, suggesting a co-regulatory mechanism involving both miR-434-3p and DNA methylation in the recovery of locomotor function after SCI.

• 1
  miR-434-3p directly targets eIF5A1 to negatively regulate its expression in rat skeletal muscle.
• 2
  Down-regulation of miR-434-3p after SCT leads to increased eIF5A1 expression, which promotes AChRs formation and improves locomotor function.
• 3
  DNA hypomethylation contributes to the up-regulation of eIF5A1 expression in hindlimb muscles after SCT.