Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability

This study explores a new approach to bone regeneration by using lactoferrin-anchored mesoporous silica nanomaterials with tannic acid (LF/TA-MSNs). These nanomaterials are designed to enhance the osteo-differentiation ability of adipose-derived stem cells (ADSCs). The LF/TA-MSNs were tested for their ability to promote bone formation by assessing alkaline phosphatase (ALP) levels, calcium accumulation, and the expression of specific genes related to bone development (osteo-differentiation-specific genes). The results suggest that LF/TA-MSNs could be a promising nanovehicle for bone healing and regeneration, especially in patients with bone defects or diseases, due to their ability to improve osteo-differentiation of ADSCs.

• 1
  LF/TA-MSNs demonstrated a prolonged release of lactoferrin (LF) for up to 28 days, indicating their potential for long-term drug delivery.
• 2
  Treatment with LF (50 µg)/TA-MSNs significantly increased alkaline phosphatase (ALP) levels and calcium accumulation in ADSCs compared to treatment with bare MSNs or lower concentrations of LF/TA-MSNs.
• 3
  LF (50 µg)/TA-MSNs notably increased the mRNA levels of osteo-differentiation-specific genes, such as osteocalcin (OCN) and osteopontin (OPN), suggesting enhanced bone formation.