Inhibition of lncRNA H19/miR-370-3p pathway mitigates neuronal apoptosis in an in vitro model of spinal cord injury (SCI)

Translational Neuroscience, 2021 · DOI: https://doi.org/10.1515/tnsci-2021-0013 · Published: January 27, 2021

Simple Explanation

Spinal cord injury (SCI) often leads to limb dysfunction, and current treatments are not always effective. Gene therapy is being explored as a new research direction. This study investigates the role of lncRNA H19 and miR-370-3p in SCI, showing that inhibiting lncRNA H19 reduces nerve cell death in a lab model. The research suggests that gene therapy targeting this pathway could potentially be used to treat SCI in the future.

Study Duration

7 weeks

Participants

12 male Sprague-Dawley rats

Evidence Level

In vitro study

Key Findings

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SCI leads to increased lncRNA H19 and decreased miR-370-3p expression.
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Inhibiting lncRNA H19 reverses LPS-induced changes, such as decreased cell viability and increased apoptosis.
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LncRNA H19 inhibition can block the NF-κB pathway through miR-370-3p upregulation.

Research Summary

This study investigates the role of lncRNA H19 and miR-370-3p in spinal cord injury (SCI) using an in vitro model. The results indicate that inhibiting lncRNA H19 can mitigate spinal neuron apoptosis, potentially through the upregulation of miR-370-3p and blockage of the NF-κB pathway. The findings suggest that targeting the lncRNA H19/miR-370-3p pathway could offer a new therapeutic strategy for SCI.

Practical Implications

Therapeutic Target

LncRNA H19/miR-370-3p pathway can be a potential target for gene therapy in SCI.

Clinical Applications

Provides possibility for clinical use of gene therapy.

Diagnostic Marker

LncRNA H19 could serve as a marker of SCI diagnosis and poor prognosis.

Study Limitations

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