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  4. Improvement of motor function in mice after implantation of mononuclear stem cells from human umbilical cord and placenta blood after 3 and 6 weeks of experimental spinal cord injury

Improvement of motor function in mice after implantation of mononuclear stem cells from human umbilical cord and placenta blood after 3 and 6 weeks of experimental spinal cord injury

Clinics, 2024 · DOI: https://doi.org/10.1016/j.clinsp.2024.100509 · Published: January 1, 2024

Spinal Cord InjuryRegenerative MedicineNeurology

Simple Explanation

This study investigates the potential of stem cell therapy to improve motor function in mice with spinal cord injuries in the chronic phase. Mononuclear stem cells from human umbilical cord and placenta blood were implanted into mice at 3 and 6 weeks post-injury to assess nerve regeneration and motor recovery. The study focuses on the early chronic phase of spinal cord injury, a period where neuronal scar formation is not yet complete, offering a window for potential recovery. By using stem cells, the researchers aim to modulate the immune response, promote neurological recovery, and stimulate angiogenesis. The findings suggest that stem cell implantation in the chronic phase can indeed provide functional and histopathological benefits, with earlier intervention (3 weeks post-injury) yielding better results than later intervention (6 weeks post-injury). These results shed light on the potential therapeutic time window for stem cell therapy in chronic spinal cord injuries.

Study Duration
12 weeks
Participants
48 Balb C mice
Evidence Level
Not specified

Key Findings

  • 1
    The intervention groups (stem cells) showed statistically significant motor improvement compared to control groups.
  • 2
    Histopathological analysis revealed that the intervention groups had a lower degree of injury (p < 0.05).
  • 3
    Intervention groups displayed increased axonal budding in the caudal portion (p < 0.05), indicating nerve regeneration.

Research Summary

This study investigates the use of mononuclear stem cells from human umbilical cord and placenta blood to improve motor function in mice with spinal cord injuries in the chronic phase. The results indicate that stem cell implantation at 3 and 6 weeks post-injury leads to functional and histopathological benefits, with earlier intervention showing better outcomes. The findings suggest a potential therapeutic time window in the chronic phase for stem cell therapy, highlighting the importance of further research in this area.

Practical Implications

Clinical Translation

The study supports further investigation of stem cell therapy in human chronic spinal cord injury patients, particularly in the early chronic phase.

Therapeutic Timing

The results suggest that the timing of stem cell intervention is critical, with earlier treatment potentially leading to better outcomes.

Stem Cell Source

The use of human umbilical cord and placenta blood as a readily available source of stem cells offers a practical advantage for clinical applications.

Study Limitations

  • 1
    Small sample size
  • 2
    Limited post-injury time evaluation
  • 3
    Lack of imaging and immunohistochemistry analysis

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