Fabrication of growth factor- and extracellular matrix-loaded, gelatin-based scaffolds and their biocompatibility with Schwann cells and dorsal root ganglia

Biomaterials, 2012 · DOI: 10.1016/j.biomaterials.2012.07.028 · Published: November 1, 2012

Simple Explanation

Researchers created gelatin-based tubes and membranes to help repair spinal cord injuries. These scaffolds were designed to protect implanted cells and release growth factors. The gelatin membranes were tested with Schwann cells and dorsal root ganglia. The membranes allowed Schwann cells to survive and effectively release growth factors. The study found that these gelatin scaffolds, which combine cross-linked gelatin technology with neurotrophins and extracellular matrix components, show promise for spinal cord repair.

Study Duration

21 days (in vitro)

Participants

Schwann cells and dorsal root ganglia from rats

Evidence Level

In vitro study

Key Findings

1
Gelatin membranes with rhBDNF improved Schwann cell adhesion, alignment, and proliferation.
2
Dorsal root ganglion explants on membranes containing laminin, fibronectin, and both neurotrophins exhibited lengthier neurite outgrowth.
3
Gelatin membranes allowed sustained release of neurotrophins (rhBDNF and rhNT-3) over 21 days.
4
Mechanical testing showed that the ultimate load was about ~0.5 N

Research Summary

The study details the fabrication of gelatin tubes and membranes for spinal cord repair, focusing on improving mechanical properties and biocompatibility. In vitro studies showed that the gelatin membranes support Schwann cell survival, promote neurite outgrowth from dorsal root ganglia, and allow for sustained release of neurotrophins. The combination of cross-linked gelatin technology with neurotrophins and extracellular matrix components demonstrates promising potential for spinal cord repair strategies.

Practical Implications

Spinal Cord Injury Treatment

The gelatin scaffolds could be used to deliver cells, growth factors, and ECM to the injured spinal cord to promote axonal regeneration and improve cell survival.

Drug Delivery Systems

The gelatin membranes can be used as a drug delivery system for local and sustained release of neurotrophins.

Tissue Engineering Applications

The gelatin scaffolds can be used as a platform for tissue engineering applications, such as nerve regeneration and cartilage repair.

Study Limitations

1
In vitro study; results may not directly translate to in vivo conditions.
2
Mechanical testing did not reveal statistical differences among groups
3
The long-term effects and biocompatibility of the gelatin scaffolds in vivo were not fully evaluated in this study.

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