Experimental study of M2 microglia transplantation promoting spinal cord injury repair in mice

Spinal cord injury (SCI) is a severe central nervous system injury. Cell transplantation is a new method for SCI treatment, and transplanted cells can improve the microenvironment of the injured area through self-differentiation and immune regulation functions. M2 microglia (MG) can secrete anti-inflammatory cytokines or chemokines to inhibit excessive inflammatory response and promote wound healing. Transplanting M2-MG induced in this way into SCI mice can effectively promote the recovery of motor function in mice. This study aims to induce MG to differentiate into M2 subtype in vitro with IL-4 and apply it to the SCI injury area of mice to evaluate its impact on neuron axon regeneration and nerve function recovery and its mechanism of action.

• 1
  M2-MG promoted axon growth when co-cultured with DRGs in vitro.
• 2
  The SCI+M2-MG group had significantly higher BMS scores than the SCI group at 21 and 28 days, and the dragging gait significantly improved at 28 days, but it did not reach the level of the sham group.
• 3
  Compared with the SCI group, the SCI+M2-MG group had a smaller injury area at 7, 14, and 28 days, an increase in neuronal survival at 28 days, and a decrease in the number of A1 astrocytes at 7 and 14 days, with significant differences.