Comprehensive Proteomic Profiling of Pressure Ulcers in Patients with Spinal Cord Injury Identifies a Specific Protein Pattern of Pathology

ADVANCES IN WOUND CARE, 2020 · DOI: 10.1089/wound.2019.0968 · Published: May 1, 2020

Spinal Cord Injury Bioinformatics Dermatology

Simple Explanation

This study focuses on the proteomic analysis of pressure ulcers (PUs) to identify changes in protein abundance during PU formation. The approach compares severe PU tissue from spinal cord injury (SCI) patients with control tissue from the same patients to define the protein pattern of this pathology. The conclusion suggests that differential abundance proteins, mainly involved in tissue regeneration, might be considered as future therapeutic options to enhance physiological response and permit cellular repair.

Study Duration

Not specified

Participants

23 subjects with SCI

Evidence Level

Not specified

Key Findings

1
The study identified 76 proteins with differences in abundance between PU and control tissue.
2
Ten proteins were verified as proteins that define the pathology: antithrombin-III, alpha-1-antitrypsin, kininogen-1, alpha-2-macroglobulin, fibronectin, apolipoprotein A-I, collagen alpha-1 (XII) chain, haptoglobin, apolipoprotein B-100, and complement factor B.
3
The proteins described in this article may have utility as new therapeutic options by supplementation, which may enhance the physiological response of wound healing.

Research Summary

This study analyzes differential protein abundance of PU tissue from SCI patients using high-throughput protein identification and quantification by TMT followed by LC-MS/MS. The study identified 76 proteins with differences in relative abundance between PU and control tissue using TMT labeling, with a subset of 20 proteins implicated in enzyme regulatory activity. A group of 10 proteins, including 6 related to enzymatic regulation, was confirmed by SRM and/or western blotting, suggesting their potential as therapeutic options to enhance wound healing.

Practical Implications

Potential Therapeutic Targets

The identified proteins may serve as potential therapeutic targets for enhancing the physiological response of wound healing in pressure ulcers.

Improved Treatment Management

The identification of new protein patterns involved in the pathogenesis of PUs may lead to better treatment management.

New Therapeutic Interventions

The findings may lead to new therapeutic interventions for PUs in SCI patients.

Study Limitations

1
Relatively small number of samples obtained.
2
Inclusion of two types of PUs in the study, grades 3 and 4.
3
Needed further studies to prove the panel of biomarkers finding in this work and with higher sample size.

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