Bioactive Three-Dimensional Chitosan-Based Scaffolds Modified with Poly(dopamine)/CBD@Pt/Au/PVP Nanoparticles as Potential NGCs Applicable in Nervous Tissue Regeneration—Preparation and Characterization

Molecules, 2024 · DOI: 10.3390/molecules29225376 · Published: November 14, 2024

Simple Explanation

This research focuses on creating new 3D materials from chitosan, a substance derived from fungi, to help regenerate damaged nerve tissue. These materials, called nerve guide conduits (NGCs), are enhanced with ingredients like poly(dopamine) and cannabidiol (CBD) to boost their bioactivity. The NGCs were made using a microwave-assisted method and then tested to see how they interact with cells. The goal is to develop materials that are biocompatible and can support nerve cell growth, potentially improving treatments for neurological conditions. The synthesized scaffolds exhibited beneficial features, confirming their potential for use in tissue engineering aimed at nervous tissue regeneration.

Study Duration

144 h

Participants

1321N1 human astrocytoma cell line

Evidence Level

Not specified

Key Findings

1
The addition of poly(dopamine) and CBD significantly impacted the scaffolds’ swelling properties and structural stability.
2
The Asp 0.84:0 samples, regardless of modification, demonstrated the highest stability and resistance to biodegradation in SBF, suggesting their potential for long-term mechanical support during tissue regeneration.
3
Cytotoxicity assessments of the scaffolds indicated that nearly all samples, both with poly(dopamine) and CBD, were biocompatible, demonstrating no negative impact on cell viability.

Research Summary

This study aimed to develop and characterize three-dimensional chitosan-based cellular scaffolds for nervous tissue regeneration, utilizing chitosan, l-Aspartic acid, l-Glutamic acid, propylene glycol, poly(dopamine) and CBD. FT-IR spectroscopy revealed molecular interactions between chitosan and the amino acids, with the presence of new bands confirming the successful incorporation of poly(dopamine) onto the chitosan surface. Swelling and biodegradation tests assessed scaffold behavior in simulated body conditions, confirming biocompatibility and safety, with cytotoxicity assessments indicating that nearly all samples, with poly(dopamine) and CBD, were biocompatible.

Practical Implications

Enhanced Nerve Regeneration

The developed scaffolds, modified with poly(dopamine) and CBD, show promise for promoting nerve cell growth and regeneration, potentially leading to improved treatments for nerve injuries.

Biocompatible Materials

The study confirms the biocompatibility of the chitosan-based scaffolds, ensuring they are safe for use in biomedical applications without causing harmful effects to cells.

Drug Delivery Systems

The swelling properties of the scaffolds can be utilized for controlled drug release, enabling targeted delivery of therapeutic agents to the site of nerve injury.

Study Limitations

1
Rapid degradation of samples containing CBD.
2
The need for further optimization of scaffold composition and preparation methods to ensure structural stability over time.
3
SBF studies do not perfectly mimic biological conditions

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