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  4. A tenascin-C mimetic peptide amphiphile nanofiber gel promotes neurite outgrowth and cell migration of neurosphere-derived cells

A tenascin-C mimetic peptide amphiphile nanofiber gel promotes neurite outgrowth and cell migration of neurosphere-derived cells

Acta Biomater, 2016 · DOI: 10.1016/j.actbio.2016.04.010 · Published: June 1, 2016

NeurologyBiomedical

Simple Explanation

This study explores a new material that mimics a part of the body's support structure, specifically a molecule called tenascin-C. This molecule is known to help nerve cells grow. The material is made of small building blocks that assemble themselves into tiny fibers. These fibers can form a gel, which can hold cells. The scientists found that when nerve cells were placed in this gel, they grew better and moved more effectively. This new gel could potentially be used to help the nervous system repair itself after injury, by guiding new nerve cells to grow and connect in the damaged area.

Study Duration
Not specified
Participants
P19 embryonal carcinoma cells, embryonic mouse neural progenitor cells
Evidence Level
Not specified

Key Findings

  • 1
    The tenascin-C mimetic PA (TN-C PA) was found to self-assemble into supramolecular nanofibers and was incorporated through co-assembly into PA gels formed by highly aligned nanofibers.
  • 2
    TN-C PA content in these gels increased the length and number of neurites produced from neurons differentiated from encapsulated P19 cells.
  • 3
    Gels containing TN-C PA were found to increase migration of cells out of neurospheres cultured on gel coatings.

Research Summary

This work demonstrates that a peptide sequence taken from tenascin-C can be incorporated into a self-assembling PA nanofiber system while retaining its neurite growth-promoting properties. Inclusion of the tenascin-C PA in gels effectively signaled neurons to increase their neurite length and increase the percentage of β-III-tubulin-positive cells relative to backbone PA gels. A significant increase in cell migration occurred on coatings composed of PA mixtures containing both the backbone PA and the TN-C PA compared to backbone PA alone.

Practical Implications

Spinal Cord Injury Therapy

Bioactive gels could serve as artificial matrix therapies in regions of neuronal loss to guide neural stem cells and promote through biochemical cues neurite extension after differentiation.

Neural Stem Cell Guidance

A material capable of guiding cells derived from neural progenitors or stem cells could be used to direct native or transplanted cells to regions of neuronal loss.

Drug Delivery Systems

The self-assembling nature of the material is the ability to transition from a solution to gel upon exposure to physiological divalent cations, like Ca2+.

Study Limitations

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