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  4. A histological comparison of the original and regenerated tail in the green anole, Anolis carolinensis

A histological comparison of the original and regenerated tail in the green anole, Anolis carolinensis

Anat Rec (Hoboken), 2012 · DOI: 10.1002/ar.22537 · Published: October 1, 2012

Regenerative MedicinePhysiologyResearch Methodology & Design

Simple Explanation

This study compares the tissues of original and regenerated lizard tails to understand regeneration. It provides baseline data for future research. The regenerated tail has a cartilage tube enclosing a spinal cord with an ependymal core, but lacks dorsal root ganglia and peripheral nerves regeneration. The muscles in the regenerated tail are disorganized with more connective tissue compared to the original tail.

Study Duration
60-90 dpa
Participants
Male and female adult A. carolinensis lizards
Evidence Level
Not specified

Key Findings

  • 1
    The regenerated tail has a cartilage tube with foramina, enclosing a spinal cord with an ependymal core, but no regeneration of dorsal root ganglia or peripheral nerves.
  • 2
    The original tail has muscle groups arranged in quadrants, while the regenerated tail has irregular muscle bundles with increased connective tissue.
  • 3
    The muscles in the regenerated tail attach to the cartilage tube and to each other, unlike the original tail where muscles attach to vertebrae.

Research Summary

This study provides a histological comparison of the mature regenerated and original tail of the lizard Anolis carolinensis. The regenerated tail has a cartilage skeleton enclosing a spinal cord but lacks dorsal root ganglia or peripheral nerves. The regenerated tail has irregular muscle bundles with increased connective tissue, differing significantly from the original tail's organized muscle groups.

Practical Implications

Understanding Regeneration

Provides a framework for future studies on lizard tail regeneration, potentially informing mammalian regeneration research.

Biomechanical Studies

The structural differences between original and regenerated tails provide a basis for biomechanical studies to understand functional capabilities and limitations.

Neuromuscular Assays

The differences in nerve regeneration can inform neuromuscular assays to understand the functional capabilities of the regenerated tail.

Study Limitations

  • 1
    Focus on a single lizard species (Anolis carolinensis)
  • 2
    Limited information on the molecular mechanisms of regeneration
  • 3
    Lack of functional studies to correlate histological findings with behavior

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