Transcription Factors STAT3 and MYC Are Key Players of Human Platelet Lysate-Induced Cell Proliferation

Human platelet lysate (HPL) is a good substitute for animal serum and helps stromal cells grow a lot. This study looks at how HPL affects genes related to the cell cycle in human stromal cells. It also tries to find the key molecules that help HPL make cells grow faster. The study found that HPL makes cell cycle genes more active in stromal cells. Since HPL has lots of growth factors, the researchers used inhibitors that block tyrosine kinase receptor (TKR) pathways, which are activated by these growth factors, and they could slow down cell growth. The research shows that HPL boosts cell growth partly by increasing TKR signaling. This then increases the production of STAT3 and MYC, which in turn activate genes that control the cell cycle.

• 1
  HPL significantly affected cell cycle-specific protein expression compared to FBS-based culture, with more genes being upregulated than downregulated.
• 2
  Blocking specific tyrosine kinase receptor (TKR) pathways lead to a significant reduction of HPL-induced cell proliferation.
• 3
  STAT3 and MYC expression and activity seem to be elevated in HPL-based cell culture, and inhibiting STAT3 significantly decreased HPL-mediated proliferation of stromal cells.