Protocol for recording the discharge of locus coeruleus neurons in free-moving mice during different sleep-wake stages

STAR Protocols, 2021 · DOI: https://doi.org/10.1016/j.xpro.2021.100981 · Published: December 17, 2021

Simple Explanation

This protocol details how to use cortical EEG combined with in vivo multichannel recording to examine the activity of locus coeruleus (LC) neurons in free-moving mice at different sleep-wake stages. The procedures include electrode fabrication, the surgery to implant electrodes, and post-recording data analysis. The technology involves EEG-EMG recording combined with in vivo multichannel recording to observe neuronal activity during different sleep-wake stages in free-moving mice, revealing that the locus coeruleus (LC) neurons are more active during wakefulness.

Study Duration

Not specified

Participants

Adult male C57BL/6N mice (6–8 weeks old)

Evidence Level

Not specified

Key Findings

1
The study found that 52.1% of the channels can be successfully recorded to the LC unit.
2
LC neurons exhibit a higher ﬁring rate during wakefulness than during NREM and REM sleep.
3
No differences were found in the mean ﬁring rates of LC neurons between NREM and REM sleep.

Research Summary

This protocol details a method for using cortical EEG combined with in vivo multichannel recording to examine the activity of locus coeruleus (LC) neurons in free-moving mice at different sleep-wake stages. It includes procedures for electrode fabrication, surgery to implant electrodes, and post-recording data analysis. The study also presents findings on the activity of LC neurons during different sleep-wake stages, noting a higher firing rate during wakefulness compared to NREM and REM sleep.

Practical Implications

Improved Understanding of Sleep-Wake Regulation

This protocol provides a valuable tool for researchers studying the neural mechanisms underlying sleep-wake regulation, particularly the role of LC neurons.

Enhanced Accuracy in Neuronal Recording

The detailed procedures for electrode fabrication and implantation can help improve the accuracy and reliability of in vivo multichannel recordings.

Advancements in Neurological Disorder Research

By enabling precise monitoring of neuronal activity during different behavioral states, this protocol can contribute to a better understanding of neurological disorders affecting sleep and arousal.

Study Limitations

1
Homemade multichannel electrodes have only 12 channels to record neuronal spikes, limiting the number of neurons recorded per mouse.
2
Electrodes are unable to be adjusted, so we cannot record the discharges of neurons in different layers of the nucleus in one experiment.
3
Electrode bundle may be too large to exclude the possibility of recording neurons outside; hence, this electrode bundle may not as favorable as silicon electrodes.

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