Inhibitor of DNA binding 2 accelerates nerve regeneration after sciatic nerve injury in mice

Neural Regen Res, 2021 · DOI: https://doi.org/10.4103/1673-5374.313054 · Published: April 23, 2021

Regenerative Medicine Neurology Genetics

Simple Explanation

This study investigates whether Inhibitor of DNA binding 2 (Id2) can promote axonal regeneration and functional recovery after peripheral nerve injury. The researchers used a mouse model of bilateral sciatic nerve crush injury and injected AAV9-Id2-3×Flag-GFP into the bilateral ventral horn of the lumbar spinal cord. The results suggest that Id2 can accelerate axonal regeneration, promote neuromuscular reinnervation, and enhance functional improvement following sciatic nerve injury.

Study Duration

2 weeks and 1 month

Participants

37 adult female C57BL/6 mice

Evidence Level

Not specified

Key Findings

1
Id2 was successfully delivered into spinal cord motor neurons projecting to the sciatic nerve.
2
The number of regenerated motor axons in the sciatic nerve distal to the crush site was increased at 2 weeks after injury.
3
The amplitude of compound muscle action potentials of the gastrocnemius muscle was markedly recovered, and their latency was shortened.

Research Summary

This study demonstrates that Id2 can accelerate axonal regeneration, promote neuromuscular reinnervation, and enhance functional improvement following sciatic nerve injury in mice. The researchers used intraspinal microinjection of AAV9 to deliver Id2 and GFP into motor neurons projecting to the sciatic nerve, enabling quantitative assessment of axonal regeneration. The findings suggest that elevating the level of Id2 in adult neurons may present a promising strategy for peripheral nerve repair following injury.

Practical Implications

Therapeutic Potential

Elevating Id2 levels in adult neurons could be a promising strategy for peripheral nerve repair after injury.

Drug Development

Id2 may serve as a target for the development of new drugs aimed at promoting nerve regeneration and functional recovery.

Further Research

Further work is required to determine whether elevation of Id2 after peripheral nerve injury can also promote axonal regeneration and functional recovery.

Study Limitations

1
The study was conducted in mice, and results may not be directly applicable to humans.
2
Further work is required to determine whether elevation of Id2 after peripheral nerve injury can also promote axonal regeneration and functional recovery.
3
The precise mechanisms by which Id2 promotes axonal regeneration in peripheral nerves remain to be fully elucidated.

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