Heat shock cognate protein 70 promotes the differentiation of C2C12 myoblast and targets Yin Yang 1

This study explores the role of Heat shock cognate protein 70 (HSC70) in skeletal muscle differentiation, specifically using C2C12 myoblasts. The researchers found that HSC70 is up-regulated during myoblast differentiation and that its knockdown inhibits differentiation. Overexpression of Yin Yang 1 (YY1) can restore differentiation in cells with HSC70 knockdown. The findings suggest HSC70's critical role in myoblast differentiation via YY1, proposing it as a therapeutic target for muscle atrophy.

• 1
  HSC70 expression is significantly up-regulated during C2C12 myoblast differentiation.
• 2
  Knockdown of HSC70 inhibits C2C12 myoblast differentiation and reduces the expression of MyoD and MyoG.
• 3
  Overexpression of Yin Yang 1 (YY1) protein promotes myoblast differentiation and restores differentiation in cells with HSC70 knockdown or inhibition.