HDAC Inhibitor Titration of Transcription and Axolotl Tail Regeneration

The study investigates how a drug, romidepsin, affects tail regeneration in axolotl embryos by influencing gene activity. Different concentrations of romidepsin were tested to find a threshold where regeneration either succeeds or fails. The researchers examined which genes were turned on or off at different drug concentrations to understand how romidepsin changes the regeneration process. They identified genes linked to oxidative stress, cell signaling, cell cycle, and cell differentiation that were affected by romidepsin. Using advanced techniques (single-nuclei RNA sequencing), they found that romidepsin's impact on gene expression occurred across various cell types, suggesting a broad mechanism regulating regeneration success or failure.

• 1
  Romidepsin inhibits axolotl embryo tail regeneration above a threshold concentration.
• 2
  Regeneration inhibitory concentrations of romidepsin increased the expression of genes associated with oxidative stress, negative regulation of cell signaling and cell cycle progression, and cellular differentiation.
• 3
  Single-nuclei RNA-Seq revealed that key genes were altered by romidepsin in the same direction across multiple cell types.