Crosstalk between m6A mRNAs and m6A circRNAs and the time-specific biogenesis of m6A circRNAs after OGD/R in primary neurons

Epigenetics, 2023 · DOI: https://doi.org/10.1080/15592294.2023.2181575 · Published: February 7, 2023

Simple Explanation

Cerebral ischemia-reperfusion injury is a pathological process where neurons experience oxygen-glucose deprivation and reoxygenation (OGD/R). The study explores m6A modifications in neurons during OGD/R. N6-methyladenosine (m6A) is the most abundant RNA modification. The study analyzes m6A modification profiles of mRNA and circRNA in normal and OGD/R-treated neurons. The study found crosstalk between m6A mRNAs and m6A circRNAs. Distinct OGD/R treatments induced the same genes to generate different m6A circRNAs, with time-specific biogenesis during OGD/R processes.

Study Duration

Not specified

Participants

Primary cerebral neurons harvested from C57BL6 mice

Evidence Level

Not specified

Key Findings

1
Expression analysis revealed that the m6A levels did not affect m6A mRNA or m6A circRNA expression in neurons.
2
The study identified three patterns of m6A circRNA production in neurons.
3
m6A circRNA biogenesis during distinct OGD/R processes was found to be time specific.

Research Summary

The study investigates m6A modification profiles in mRNAs and circRNAs of normal and OGD/R-treated neurons to understand epigenetic mechanisms involved in cerebral ischemia-reperfusion injury. The research revealed that m6A levels do not affect the expression of m6A mRNAs or circRNAs but found evidence of crosstalk between them and identified three patterns of m6A circRNA production. The study also discovered that the production of m6A circRNAs is time-specific, expanding the understanding of m6A modifications in normal and OGD/R-treated neurons.

Practical Implications

Potential Therapeutic Targets

Understanding m6A modification patterns may lead to the development of targeted therapies for OGD/R-related diseases.

Epigenetic Insights

The study provides a reference for exploring epigenetic mechanisms involved in neuronal injury and recovery.

Time-Specific Interventions

The time-specific nature of m6A circRNA biogenesis suggests that interventions may need to be tailored to specific stages of OGD/R injury.

Study Limitations

1
The exact mechanisms by which m6A modifications influence neuronal function require further investigation.
2
The study primarily focuses on primary neurons in vitro, and in vivo validation is needed.
3
The cell-type-specificity of m6A modifications needs further elucidation.

Your Feedback

Was this summary helpful?

Back to Neurology