eNeuro, 2019 · DOI: https://doi.org/10.1523/ENEURO.0025-19.2019 · Published: March 28, 2019
Researchers created a new mouse model to selectively edit the genes of injured neurons. They used a Cre-ERT2 construct driven by ATF3, a marker of nerve damage, to activate gene editing only in injured neurons. When crossed with reporter mice, recombination occurred only in injured neurons following sciatic nerve injury or spinal hemisection, indicating that the system works as intended. This new tool allows scientists to study the genetic control of injury responses and regeneration in a more targeted way, by editing genes only in the neurons that are affected by the injury.
The ATF3-CreERT2 mouse line enables precise genetic manipulation of injured neurons, allowing researchers to investigate specific genes involved in injury responses and regeneration.
By selectively editing the genome of injured neurons, this model can help elucidate the molecular mechanisms underlying neurotrauma and identify potential therapeutic targets.
The ability to enhance axonal regeneration through targeted gene excision, such as PTEN, offers new avenues for developing strategies to promote nerve repair and functional recovery after injury.