Amputation-Induced ROS Signaling is Required for Axolotl Tail Regeneration

Salamanders possess a unique ability to regenerate lost limbs and tails. This study investigates the early signals that trigger this regeneration process in axolotls, focusing on the role of reactive oxygen species (ROS). The research found that ROS production increases rapidly after tail amputation in axolotl embryos and remains elevated for 24 hours. Blocking ROS production with specific inhibitors reduced cell proliferation and inhibited tail regeneration. These findings suggest that ROS act as crucial early signals, possibly initiating cues, that are necessary for successful tail regeneration in salamanders.

• 1
  ROS levels increase rapidly following tail amputation in axolotl embryos, peaking within minutes and remaining elevated for 24 hours.
• 2
  Pharmacological inhibition of ROS-producing enzymes (using DPI and VAS2870) reduces ROS levels and inhibits tail outgrowth.
• 3
  ROS signaling and NOX activity are essential for cell cycle re-entry and proliferation, particularly in the spinal cord, during tail regeneration.