Silencing the gene encoding C/EBP homologous protein lessens acute brain injury following ischemia/reperfusion

The study investigates the role of C/EBP homologous protein (CHOP) in brain injury following ischemia/reperfusion. CHOP is a transcription factor involved in endoplasmic reticulum stress and cell apoptosis. The researchers used short hairpin RNA (shRNA) to silence the CHOP gene in rat models of ischemia/reperfusion. They then analyzed the effects of this silencing on brain injury. The findings suggest that silencing CHOP lessens cell apoptosis and inflammatory reactions, thereby protecting nerves after ischemia/reperfusion injury.

• 1
  Silencing C/EBP homologous protein gene expression significantly reduced cerebral infarction volume in rats following cerebral ischemia/reperfusion.
• 2
  CHOP silencing reduced tumor necrosis factor-α (TNF-α) mRNA and interleukin-1β mRNA expression in the infarct region.
• 3
  CHOP silencing increased Bcl-2 content and decreased caspase-3 content in the infarct region, indicating reduced apoptosis.